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Faculty Information

Joseph Guttenplan, M.S., Ph.D.
Professor
Basic Science and Craniofacial Biology
Room 1015S Schwartz Hall, 345 East 24Th Street
Fax: 212-995-4087
E-mail:

 

Education:

1965 B.S., Brooklyn College
1970 M.S., Brandeis University
1970 Ph.D., Brandeis University
1969-71 Postdoc Max Planck Institute for Biophysical Chemistry, Gottingen
1971-73 Postdoc University of California, Berkeley

 

Research Interests / Professional Overview:

  • Effects of nutrients on spontaneous and chemically induced mutagenesis in lacI transgenic mice.
  • Effects of nutaceuticals and tobacco on gene expression in human and rodent tissues.
  • Mutagenesis by smokeless tobacco, tobacco components, and tobacco carcinogens in lacZ transgenic mice. 
  • Detection of chemically modified DNA in human oral tissue.
  • Metabolism of tobacco carcinogens by human oral tissue.
  • Development of sensitive fluorescent assays to detect DNA damage.

Chemical Carcinogenesis and Mutagenesis
Research in my laboratory focuses on measuring mutations in tissues of lacZ transgenic mice, and cultured cells, chemoprevention of mutagenesis, development of high sensitivity fluorescence assays for detection of modified nucleotides from DNA, the metabolism of carcinogens, interactions of carcinogens with DNA, detection of carcinogen-DNA adducts, and determination of mutational spectra of carcinogens. Laboratory methodologies include high performance liquid chromatography, mutagenesis, PCR, enzyme assays, gene arrays.

 

Pub Med Articles:

Guttenplan JB

 

Representative Publications:

Kosinska, W.,  Khmelnitsky, M., Kim, J-H, Zhao, Z-L, and Guttenplan, J. G.,   Effects of Potential Dietary Inhibitors of Endogenous DNA Damage on Mutagenesis and Lipid Peroxidation in lacZ Mice, Environmental & Molecular Mutagenesis. 52, 502-9, (2011).  PMID: 21538553. 

Zhiming He, Wieslawa Kosinska, Zhong-Lin Zhao, Xue-Ru Wu, Joseph B. Guttenplan. Tissue-specific mutagenesis by N-butyl-N-(4-hydroxybutyl)nitrosamine as the basis for urothelial carcinogenesis. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 742, (1–2), 92–95 (2012). UI 22155125

Mohebati, A., Guttenplan, J.B., Kochhar, A., Zhao, Z-L, Kosinska, W., Subbaramaiah, K. and Dannenberg, A.J., Carnosol, a Constituent of Zyflamend, Inhibits Aryl Hydrocarbon Receptor-Mediated Activation of CYP1A1 and CYP1B1 Transcription and Mutagenesis. Cancer Prev. Res., 5, 593-602 (2012). PMID: 2374940.  

Guttenplan JB, Kosinska W, Zhao ZL, Chen KM, Aliaga C, Deltondo J, Cooper T, Sun YW, Zhang S, Jiang K, Bruggeman R, Sharma AK, Amin S, Ahn K, El-Bayoumy K.,  Mutagenesis and carcinogenesis induced by dibenzo[a,l]pyrene in the mouse oral cavity: A potential new model for oral cancer, Int J Cancer. 130, 2783-90 (2011) PMID: 21815141.

Sacks, P.G., Zhao, Z.L., Kosinska, W., Fleisher, K, Gordon,T., Guttenplan, J.B., Concentration dependent effects of tobacco particulates from different types of cigarettes on expression of drug metabolizing proteins, and benzo(a)pyrene metabolism in primary normal human oral epithelial cells,  Food Chem Toxicol, 49, 2348-55 (2011) PMID: 21722697.

Chen KM, Sacks PG, Spratt TE, Lin JM, Boyiri T, Schwartz J, Richie JP, Calcagnotto A, Das A, Bortner J, Zhao Z, Amin S, Guttenplan J, El-Bayoumy K. Modulations of benzo[a]pyrene-induced DNA adduct, cyclin D1 and PCNA in oral tissue by 1,4-phenylenebis(methylene)selenocyanate. Biochem Biophys Res Commun. 383, 151-5. (2009). PMID: 19344691

Nair, S., Kekatpure, V.D. Judson, B.L., Rifkind, A.B., Granstein, R.D., Boyle, J.O., Subbaramaiah, K., Guttenplan, J.B., and Dannenberg, A.J., UVR Exposure Sensitizes Keratinocytes to DNA Adduct Formation, Cancer Prev. Res., 2, 895 - 902 (2009). PMCID: PMC2758323.

Santen, R., Cavalieri, E., Rogan, E., Russo, J., Guttenplan,J.B., Ingle, J., and Yue,W., Estrogen mediation of breast tumor formation involves estrogen receptor dependent as well independent, genotoxic effects, Ann. N.Y. Acad. Sci., 1155,132-40 (2009). PMID: 19250200.

Sun YW, Guttenplan JB, Khmelnitsky M, Krzeminski J, Boyiri T, Amin S, El-Bayoumy K., Stereoselective metabolism of the environmental mammary carcinogen 6-nitrochrysene to trans-1,2-dihydroxy-1,2-dihydro-6-nitrochrysene by aroclor 1254-treated rat liver microsomes and their comparative mutation profiles in a laci mammary epithelial cell line. Chem Res Toxicol.,12, 1992-7 (2009). PMID: 19886636

Hughes, D., Guttenplan, J.B., Marcus, C.B., Subbaramaiah, K., and Dannenberg, A.J., HSP90 Inhibitors Suppress Aryl Hydrocarbon Receptor-Mediated Activation of CYP1A1 and CYP1B1 Transcription and DNA Adduct Formation. Cancer Prev. Res., 1, 485-93 (2008). PMCID: PMC2680610.

Guttenplan, J.B., Chen, K-M., Khmelnitsky, M., Kosinska, W., Hennessy, J, Bruggeman, R., Desai, D., Amin, S., Sun, Y-W., Spratt, T., and El-Bayoumy, K., Effects of 1,4-phenylenebis(methylene)selenocyanate on mutagenesis and p53 protein expression in the tongue of lacI rats treated with 4-nitroquinoline-N-oxide, Mutation Res., 634, 146-155 (2007). PMCID: PMC2700054.

Guttenplan, J.B., Chen, K-M., Khmelnitsky, M., Kosinska, W., Hennessy, J, Bruggeman, R., Desai, D., Amin, S., Sun, Y-W., Spratt, T., and El-Bayoumy, K., Effects of 1,4-phenylenebis(methylene)selenocyanate on mutagenesis and p53 protein expression in the tongue of lacI rats treated with 4-nitroquinoline-N-oxide, Mutation Res., 634, 146-155 (2007). PMCID: PMC2700054.

Zhao, Z., Kosinska, W., Khmelnitsky, M., Cavalieri, E.L., Rogan, E.G., Chakravarti, D., Sacks, P.G., and Guttenplan, J.B., Mutagenic activity of 4-hydroxyestradiol, but not 2-hydroxyestradiol, in BB® Rat2 embryonic cells, and the mutational spectrum of 4-hydroxyestradiol, Chem. Res. Tox. 19, 475-479 (2006). PMC Journal – In Process

Cavalieri, E., Chakravarti, D., Guttenplan, J.B., Jankowiak, R., Rogan, E., Russo, J., Santen, R., and Sutter, T., Catechol Estrogen Quinones as Initiators of Breast and Other Human Cancers. Implications for Cancer Prevention and Biomarkers of Susceptibility, BBA, 1766, 63-78 (2006).