Dr. Malamud specializes in the development of anti-HIV agents and oral-based diagnostics. The area of oral-based diagnostics is a rapidly evolving field that has developed from the realization that most molecules present in blood can also be detected within the oral cavity, thus providing a relatively non-invasive diagnostic method. Current studies focus on a point-of-care microfluidic cassette that can detect multiple analytes present in bacterial and viral pathogens. A second project deals with alterations in the gastrointestinal track following administration of antiviral therapy to HIV+ individuals. In this study research teams are monitoring proteomics, microbiomics and alterations in the innate immune system. Dr. Malamud's research, which has been continuously funded by the NIH for over 30 years, deals with HIV pathogenesis, the design of anti-HIV drugs, and novel diagnostics using oral samples. His investigations in these areas have involved human salivary proteins that inhibit HIV infectivity and the discovery and development of anti-HIV compounds that can be used to prevent HIV infection.
Malamud D, Wahl SM. 2010. The mouth: a gateway or a trap for HIV? AIDS 2;24(1):5-16
Stoddard E, Ni H, Cannon G, Zhou C, Kallenbach N, Malamud D, Weissman D. 2009. gp340 promotes transcytosis of human immunodeficiency virus type 1 in genital tract-derived cell lines and primary endocervical tissue. J Virol. 83(17):8596-603.
Liu C, Qiu X, Ongagna S, Chen D, Chen Z, Abrams WR, Malamud D, Corstjens PL, Bau HH. 2009. A timer-actuated immunoassay cassette for detecting molecular markers in oral fluids. Lab Chip. 2009 Mar 21;9(6):768-76.
Stoddard E, Cannon G, Ni H, Karikó K, Capodici J, Malamud D, Weissman D. 2007. gp340 expressed on human genital epithelia binds HIV-1 envelope protein and facilitates viral transmission. J Immunol. 1;179(5):3126-32.
Malamud, D, Niedbala, RS. 2007. Oral-based Diagnostics. Annals NY Academy of Sciences. Volume 1098.
Stoddard E, Cannon G, Ni H, Karikó K, Capodici J, Malamud D, Weissman D. (2007)
J Immunol.179(5):3126-32.PMID: 17709527 gp340 expressed on human genital epithelia binds HIV-1 envelope protein and facilitates viral transmission.
Cannon, G, Yi, (2008), Stoddard, E, Scales, DA, Van Ryk, DI, Chaiken, I, Malamud, D, Weissman, D. HIV envelope binding by macrophage-expressed gp340 promotes HIV-1 infection. J Immunol; 181:2065-2070.
Stoddard, E, Cannon, G, Ni, H, Kariko, K, Capodici, J, Malamud, D, Weissman, D. (2007) gp340 expressed on human genital epithelia binds HIV-1 envelope protein and facilitates viral transmission. J Immunol; 179:3126-3132.
Chen, Z, Mauk, MG, (2007), J, Abrams, WR, Corstjens, PL, Niedbala, RS, Malamud, D, Bau, HH. A microfluidic system for saliva-based detection of infectious diseases. Ann NY Acad Sci 1098:429-436.
Malamud, D, Abrams, WR, Bau HH, Wang, J, Chen, Z, Corstjens, P, Niedbala, RS. , (2006) Oral-based techniques for the diagnosis of infectious diseases. J Calif Dent Assoc 34(4):297-301.
Malamud, D, Bau H, Niedbala S, Corstjens P. Point detection of pathogens in oral samples. Adv Dent Res. (2005); 18(1): 12-6.
Slaughter YA, Malamud D. (2005) diagnostics for the geriatric populations: current status and future prospects. Dent Clin North Am.;49(2):445-61.
Barnhart KT, Pretorius ES, Timbers K, Shera D, Shabbout M, Malamud D. (2004) In vivo distribution of a vaginal gel: MRI evaluation of the effects of gel volume, time and simulated intercouse. Contraception. 70(6):498-505.
Holm-Hansen C, Tong G, Davis C, Abrams WR, Malamud D. Comparison of oral fluid collectors for use in a rapid point-of-care diagnostic device. Clin Diagn Lab Immunol. 2004 Sep;11(5):909-12.
Wu Z, Golub E, Abrams WR, Malamud D. gp340 (SAG) binds to the V3 sequence of gp120 important for chemokine receptor interaction. AIDS Res Hum Retroviruses. 2004 Jun;20(6):600-7.
Wu Z, Van Ryk D, Davis C, Abrams WR, Chaiken I, Magnani J, Malamud D. Salivary agglutinin inhibits HIV type 1 infectivity through interaction with viral glycoprotein 120. AIDS Res Hum Retroviruses. 2003 Mar;19(3):201-9.
Pretorius ES, Timbers K, Malamud D, Barnhart K. Magnetic resonance imaging to determine the distribution of a vaginal gel: before, during, and after both simulated and real intercourse. Contraception. 2002 Dec;66(6):443-51.
Malamud, D., Davis, C., Berthold, P., Roth, E., and Freidman, H.A. (1993) Human Submandibular Saliva Aggregates HIV. AIDS Research Human Retro. 9:633 637.
Malamud, D. and Tabak, L., (1993) Saliva as a diagnostic fluid. Annals NY Academy of Sciences Volume 694.
Pearce Pratt, R, Malamud, D. and Phillips, D.M. (1994) Role of the Cytoskeleton in Cell to Cell Transmission of Human Immunodeficiency Virus. J. Virology 68:2898 2905.