We use baker's yeast to study the process underlying meiosis and meiotic recombination. We are particularly interested in the surveillance mechanisms, or checkpoints, that provide the regulatory framework for the step-by-step progression of many meiotic events.


Spotlight


Markowitz TE, Suarez D, Blitzblau HG, Patel NJ, Markhard AL, MacQueen AJ, Hochwagen A. (2017) Reduced dosage of the chromosome axis factor Red1 selectively disrupts the meiotic recombination checkpoint in Saccharomyces cerevisiae. PLoS Genet, 13(7):e1006928


Wang D, Mansisidor A, Prabhakar G, Hochwagen A. (2016) Condensin and Hmo1 mediate a starvation-induced transcriptional position effect within the ribosomal DNA array. Cell Rep, 14:1010-1017


Subramanian VV, MacQueen AJ, Vader G, Shinohara M, Sanchez A, Borde V, Shinohara A, Hochwagen A. (2016) Chromosome synapsis alleviates Mek1-dependent suppression of meiotic DNA repair PLoS Biol, 14(2):e1002369



Research


 

Meiotic Checkpoints

The meiotic program is orchestrated by a whole network of checkpoint mechanisms. Our research combines molecular biology and systems biology to determine the components and dynamics of this network.

Meiotic chromosome structure

Meiotic chromosomes undergo profound structural changes including programmed chromosome breakage. We use genomic analyses and chromosome engineering to understand the rules governing chromosome transitions and breakage patterns.

 

Publications


 

2017

Markowitz TE, Suarez D, Blitzblau HG, Patel NJ, Markhard AL, MacQueen AJ, Hochwagen A. (2017) Reduced dosage of the chromosome axis factor Red1 selectively disrupts the meiotic recombination checkpoint in Saccharomyces cerevisiae. PLoS Genet, 13(7):e1006928 PubMed PDF

Argunhan B, Leung WK, Afshar N, Terentyev Y, Subramanian VV, Murayama Y, Hochwagen A, Iwasaki H, Tsubouchi T, Tsubouchi H. (2017) Fundamental cell cycle kinases collaborate to ensure timely destruction of the synaptonemal complex during meiosis. EMBO J, 36(17):2488-2509 PubMed

2016

Subramanian VV, MacQueen AJ, Vader G, Shinohara M, Sanchez A, Borde V, Shinohara A, Hochwagen A. (2016) Chromosome Synapsis Alleviates Mek1-Dependent Suppression of Meiotic DNA Repair. PLoS Biol, 14(2):e1002369 PubMed PDF

Wang D, Mansisidor A, Prabhakar G, Hochwagen A. (2016) Condensin and Hmo1 Mediate a Starvation-Induced Transcriptional Position Effect within the Ribosomal DNA Array. Cell Rep, 14:1010-1017 PubMed PDF

He H, Zhang S, Wang D, Hochwagen A, Li F. (2016) Condensin Promotes Position Effects within Tandem DNA Repeats via the RITS Complex. Cell Rep, 14:1018-1024 PubMed PDF

2015

Vincenten N, Kuhl LM, Lam I, Oke A, Kerr AR, Hochwagen A, Fung J, Keeney S, Vader G, Marston AL. (2015) The kinetochore prevents centromere-proximal crossover recombination during meiosis. eLife 2015;4:e10850 PubMed PDF

Gothwal SK, Patel NJ, Colletti MM, Sasanuma H, Shinohara M, Hochwagen A, Shinohara A. (2015) The Double-Strand Break Landscape of Meiotic Chromosomes Is Shaped by the Paf1 Transcription Elongation Complex in Saccharomyces cerevisiae. Genetics, 202(2):497-512 PubMed

Sun X, Huang L, Markowitz TE, Blitzblau HG, Chen D, Klein F, Hochwagen A. (2015) Transcription dynamically patterns the meiotic chromosome-axis interface. eLife 2015;4:e07424 PubMed PDF

Voelkel-Meiman K, Johnston C, Thappeta Y, Subramanian VV, Hochwagen A, MacQueen AJ. (2015) Separable Crossover-Promoting and Crossover-Constraining Aspects of Zip1 Activity during Budding Yeast Meiosis. PLoS Genet, 11(6):e1005335 PubMed PDF

2014

Humphryes N, Hochwagen A. (2014) A non-sister act: Recombination template choice during meiosis. Exp Cell Res, 15;329(1):53-60 PubMed

Subramanian VV, Hochwagen A. (2014) The Meiotic Checkpoint Network: Step-by-Step through Meiotic Prophase. Cold Spring Harb Perspect Biol 6(10):a016675 PubMed

2013

Copsey A, Tang S, Jordan PW, Blitzblau HG, Newcombe S, et al. (2013) Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions. PLoS Genet 9(12): e1004071 PubMed PDF

Blitzblau HG, Hochwagen A (2013) ATR/Mec1 prevents lethal meiotic recombination initiation on partially replicated chromosomes in budding yeast. eLife 2013;2:e00844 PubMed PDF

2012

Agarwala SD, Blitzblau HG, Hochwagen A, Fink GR (2012) RNA Methylation by the MIS Complex Regulates a Cell Fate Decision in Yeast. PLoS Genet 8(6): e1002732. PubMed PDF

Blitzblau HG, Chan CS, Hochwagen A, Bell SP (2012) Separation of DNA Replication from the Assembly of Break- Component Meiotic Chromosomes. PLoS Genet 8(5): e1002643. PubMed PDF

2011

Subramanian, V.V., and Hochwagen, A. (2011). Centromere clustering: where synapsis begins. Curr Biol 21, R920-922. PubMed

Vader, G., Blitzblau, H.G., Tame, M.A., Falk, J.E., Curtin, L., and Hochwagen, A. (2011). Protection of repetitive DNA borders from self-induced meiotic instability. Nature 477, 115-119. PubMed PDF Supplemental Data PDF

Blitzblau, H.G., and Hochwagen, A. (2011). Genome-wide detection of meiotic DNA double-strand break hotspots using single-stranded DNA. Methods Mol Biol 745, 47-63. PubMed PDF

MacQueen, A.J., and Hochwagen, A. (2011). Checkpoint mechanisms: the puppet masters of meiotic prophase. Trends Cell Biol 21, 393-400. PubMed

Pan, J., Sasaki, M., Kniewel, R., Murakami, H., Blitzblau, H.G., Tischfield, S.E., Zhu, X., Neale, M.J., Jasin, M., Socci, N.D., Hochwagen, A., Keeney, S. (2011). A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation. Cell 144, 719-731. PubMed PDF Supplemental Data PDF

2010

Falk, J.E., Chan, A.C., Hoffmann, E., and Hochwagen, A. (2010). A Mec1- and PP4-dependent checkpoint couples centromere pairing to meiotic recombination. Dev Cell 19, 599-611. PubMed

Hochwagen, A., and Marais, G.A. (2010). Meiosis: a PRDM9 guide to the hotspots of recombination. Curr Biol 20, R271-274. PubMed

2009

Hochwagen, A. (2009). Meiosis: making a synaptonemal complex just got easier. Curr Biol 19, R849-851. PubMed

Brar, G.A., Hochwagen, A., Ee, L.S., and Amon, A. (2009). The multiple roles of cohesin in meiotic chromosome morphogenesis and pairing. Mol Biol Cell 20, 1030-1047. PubMed PDF

2008

Chen, S.Y., Tsubouchi, T., Rockmill, B., Sandler, J.S., Richards, D.R., Vader, G., Hochwagen, A., Roeder, G.S., and Fung, J.C. (2008). Global analysis of the meiotic crossover landscape. Dev Cell 15, 401-415. PubMed PDF Supplemental Data PDF

Hochwagen, A. (2008). Meiosis. Curr Biol 18, R641-R645. PubMed

2007

Blitzblau, H.G., Bell, G.W., Rodriguez, J., Bell, S.P., and Hochwagen, A. (2007). Mapping of meiotic single-stranded DNA reveals double-stranded-break hotspots near centromeres and telomeres. Curr Biol 17, 2003-2012. PubMed

2006

Hochwagen, A., and Amon, A. (2006). Checking your breaks: surveillance mechanisms of meiotic recombination. Curr Biol 16, R217-228. PubMed

2005

Hochwagen, A., Tham, W.H., Brar, G.A., and Amon, A. (2005). The FK506 binding protein Fpr3 counteracts protein phosphatase 1 to maintain meiotic recombination checkpoint activity. Cell 122, 861-873. PubMed

Hochwagen, A., Wrobel, G., Cartron, M., Demougin, P., Niederhauser-Wiederkehr, C., Boselli, M.G., Primig, M., and Amon, A. (2005). Novel response to microtubule perturbation in meiosis. Mol Cell Biol 25, 4767-4781. PubMed PDF

2002

Haering, C.H., Lowe, J., Hochwagen, A., and Nasmyth, K. (2002). Molecular architecture of SMC proteins and the yeast cohesin complex. Mol Cell 9, 773-788. PubMed

2000

Panizza, S., Tanaka, T., Hochwagen, A., Eisenhaber, F., and Nasmyth, K. (2000). Pds5 cooperates with cohesin in maintaining sister chromatid cohesion. Curr Biol 10, 1557-1564. PubMed

Lab members


 

Principal investigator

 

Andreas

Andreas Hochwagen, PhD
Principal Investigator
Assistant Professor of Biology
andi@nyu.edu

 

PostDocs

Viji

Viji Subramanian, PhD
Postdoc
vvs2@nyu.edu

Research interests: Investigating the role of the synaptonemal complex in the regulation of the double-strand break repair outcome.

Luis

Luis A. Vale Silva, PhD
Postdoc
luis.valesilva@nyu.edu

Research interests: Investigating the effect of chromosome size on meiotic double-strand break formation.

Graduate Students

Tovah

Tovah Markowitz
PhD student
tem298@nyu.edu

Research interests: Understanding how condensin and cohesin control meiotic chromosome structure and recombination.

Danni

Danni Wang
PhD student
dw1258@nyu.edu

Research interests: Analyzing position effects of the repetitive rDNA array.

Andres

Andres Mansisidor
PhD student
am4992@nyu.edu

Research interests: Investigating the structural and functional dynamics of repetitive DNA and the physiological consequences of such dynamics.

Matt

Matthew Robert Paul
PhD student
mrp420@nyu.edu

Research interests: The role of the 3D organization of chromatin on meiotic double-strand break formation.

Jonna

Jonna Heldrich
PhD student
jh4321@nyu.edu

Research interests: Understanding the role of genome topology in the formation of meiotic double strand breaks.

Temi

Temistocles Molinar Jr.
PhD student
tm2484@nyu.edu

Research interests: Causes and consequences of ribosomal DNA array instability.

Victor

Funda Mujgan Kar
PhD student
fmk251@nyu.edu

Research interests: Understanding the role of posttranslational modifications in meiosis.

Charlene

Charlene Valdez
Master's student & Technician
cv1026@nyu.edu

Research interests: Control of pre-meiotic DNA replication.

Victor

Victor A. Leon
Master's student
val271@nyu.edu

Research interests: Characterization of phosphorylation of the synapsis protein Zip1.

Ninkka

Ninkka Tamot
Master's student
nt1226@nyu.edu

Research interests: Structural and functional analysis of the high-mobility group protein Hmo1.

Undergraduate students

Vivian

Vivian Utti
Undergraduate student
vu246@nyu.edu

Michelle

Michelle Shi
Undergraduate student
mss723@nyu.edu

Former lab members

Hanna

Hannah Blitzblau, PhD
Postdoc

Moved on to become
Senior Research Scientist at Novogy

Gerben

Gerben Vader, PhD
Postdoc
Moved on to become
Junior Group Leader at Max Planck Institute of Molecular Physiology (Lab website)

Neil

Neil Humphryes, PhD
Postdoc

Moved on to become
Senior Bioinformatician at Desktop Genetics, Ltd

trevor

Trevor Stoltzfus
Master's student

Moved on to become
Medical student at Des Moines University

Neem

Neem Patel
Master's student & Technician

Moved on to become
PhD student at UC Berkeley

Marie

Marie Jezequel
Visiting Master's student

Moved on to become
Product Manager at Pierre Fabre Laboratories

Jawaher

Jawaher Al-Zahrani
Research volunteer

Moved on to become
PhD student at Cornell University

Daniel

Daniel Suarez
Undergraduate student

Moved on to
Postbaccalaureate program at SUNY Purchase College

Ashima

Ashima Goel
Undergraduate student


Priyanka

Priyanka Srivastava
Undergraduate student


Anna

Anna Maria Houshangi
Master's student & Technician

Moved on to become
NYC Teaching Fellow at Department of Education

Sunny

Sunny (Xiaoji) Sun
Master's student

Moved on to become
PhD Student at NYU School of Medicine

Meaghan

Meaghan Michelle Colletti
Master's student

Moved on to become
Student at UT Southwestern Medical Center

Daeun

Daeun Hur
Undergraduate student

Moved on to become
Student at NYU College of Dentistry

Raj

Raj A. Desai
Undergraduate student

Moved on to become
Student at Columbia University College of Dental Medicine

Alisha

Alisha Karim
Undergraduate student


Raghav

Raghav Mishra
Undergraduate student


Namitha

Namitha L Gubbi
Undergraduate student


Mihoko

Mihoko Tame
Lab Manager & Technician

Moved on to become
PhD student at NKI-AVL

Jill

Jill Falk
Lab Manager & Technician

Moved on to become
PhD student at MIT

Andrew

Andrew Markhard
Lab Manager & Technician

Moved on to become
Technician at Mootha Lab in Broad Institute of MIT & Harvard

Protocols


 

Genome-wide detection of meiotic DNA double-strand break hotspots using single-stranded DNA. (PDF)

BYTA Medium Recipe. (PDF)

Dissolving Benomyl in Sporulation Medium. (PDF )

 

Lab Photos


 

Click to see all photos

Contact us

 

Andreas

 

Andreas Hochwagen, PhD
Principal Investigator
Assistant Professor of Biology
andi@nyu.edu
Lab: 1-(212)-992-6556

 

Lab mailing address

Hochwagen Lab
New York University
Department of Biology
1009 Silver Center
100 Washington Square East
New York, NY 10003-6688

Lab shipping address

Hochwagen Lab
New York University
Department of Biology
150 Brown
100 Washington Square East
New York, NY 10003

New York University    Department of Biology

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