We use baker's yeast to study the process underlying meiosis and meiotic recombination. We are particularly interested in the surveillance mechanisms, or checkpoints, that provide the regulatory framework for the step-by-step progression of many meiotic events.


Subramanian VV, MacQueen AJ, Vader G, Shinohara M, Sanchez A, Borde V, Shinohara A, Hochwagen A. (2016) Chromosome synapsis alleviates Mek1-dependent suppression of meiotic DNA repair PLoS Biol, 14(2):e1002369

Wang D, Mansisidor A, Prabhakar G, Hochwagen A. (2016) Condensin and Hmo1 mediate a starvation-induced transcriptional position effect within the ribosomal DNA array. Cell Rep, 14:1010-1017



Meiotic Checkpoints

The meiotic program is orchestrated by a whole network of checkpoint mechanisms. Our research combines molecular biology and systems biology to determine the components and dynamics of this network.

Meiotic chromosome structure

Meiotic chromosomes undergo profound structural changes including programmed chromosome breakage. We use genomic analyses and chromosome engineering to understand the rules governing chromosome transitions and breakage patterns.





Subramanian VV, MacQueen AJ, Vader G, Shinohara M, Sanchez A, Borde V, Shinohara A, Hochwagen A. (2016) Chromosome Synapsis Alleviates Mek1-Dependent Suppression of Meiotic DNA Repair. PLoS Biol, 14(2):e1002369 PubMed PDF

Wang D, Mansisidor A, Prabhakar G, Hochwagen A. (2016) Condensin and Hmo1 Mediate a Starvation-Induced Transcriptional Position Effect within the Ribosomal DNA Array. Cell Rep, 14:1010-1017 PubMed PDF

He H, Zhang S, Wang D, Hochwagen A, Li F. (2016) Condensin Promotes Position Effects within Tandem DNA Repeats via the RITS Complex. Cell Rep, 14:1018-1024 PubMed PDF


Vincenten N, Kuhl LM, Lam I, Oke A, Kerr AR, Hochwagen A, Fung J, Keeney S, Vader G, Marston AL. (2015) The kinetochore prevents centromere-proximal crossover recombination during meiosis. eLife 2015;4:e10850 PubMed PDF

Gothwal SK, Patel NJ, Colletti MM, Sasanuma H, Shinohara M, Hochwagen A, Shinohara A. (2015) The Double-Strand Break Landscape of Meiotic Chromosomes Is Shaped by the Paf1 Transcription Elongation Complex in Saccharomyces cerevisiae. Genetics, 202(2):497-512 PubMed

Sun X, Huang L, Markowitz TE, Blitzblau HG, Chen D, Klein F, Hochwagen A. (2015) Transcription dynamically patterns the meiotic chromosome-axis interface. eLife 2015;4:e07424 PubMed PDF

Voelkel-Meiman K, Johnston C, Thappeta Y, Subramanian VV, Hochwagen A, MacQueen AJ. (2015) Separable Crossover-Promoting and Crossover-Constraining Aspects of Zip1 Activity during Budding Yeast Meiosis. PLoS Genet, 11(6):e1005335 PubMed PDF


Humphryes N, Hochwagen A. (2014) A non-sister act: Recombination template choice during meiosis. Exp Cell Res, 15;329(1):53-60 PubMed

Subramanian VV, Hochwagen A. (2014) The Meiotic Checkpoint Network: Step-by-Step through Meiotic Prophase. Cold Spring Harb Perspect Biol 6(10):a016675 PubMed


Copsey A, Tang S, Jordan PW, Blitzblau HG, Newcombe S, et al. (2013) Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions. PLoS Genet 9(12): e1004071 PubMed PDF

Blitzblau HG, Hochwagen A (2013) ATR/Mec1 prevents lethal meiotic recombination initiation on partially replicated chromosomes in budding yeast. eLife 2013;2:e00844 PubMed PDF


Agarwala SD, Blitzblau HG, Hochwagen A, Fink GR (2012) RNA Methylation by the MIS Complex Regulates a Cell Fate Decision in Yeast. PLoS Genet 8(6): e1002732. PubMed PDF

Blitzblau HG, Chan CS, Hochwagen A, Bell SP (2012) Separation of DNA Replication from the Assembly of Break- Component Meiotic Chromosomes. PLoS Genet 8(5): e1002643. PubMed PDF


Subramanian, V.V., and Hochwagen, A. (2011). Centromere clustering: where synapsis begins. Curr Biol 21, R920-922. PubMed

Vader, G., Blitzblau, H.G., Tame, M.A., Falk, J.E., Curtin, L., and Hochwagen, A. (2011). Protection of repetitive DNA borders from self-induced meiotic instability. Nature 477, 115-119. PubMed PDF Supplemental Data PDF

Blitzblau, H.G., and Hochwagen, A. (2011). Genome-wide detection of meiotic DNA double-strand break hotspots using single-stranded DNA. Methods Mol Biol 745, 47-63. PubMed PDF

MacQueen, A.J., and Hochwagen, A. (2011). Checkpoint mechanisms: the puppet masters of meiotic prophase. Trends Cell Biol 21, 393-400. PubMed

Pan, J., Sasaki, M., Kniewel, R., Murakami, H., Blitzblau, H.G., Tischfield, S.E., Zhu, X., Neale, M.J., Jasin, M., Socci, N.D., Hochwagen, A., Keeney, S. (2011). A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation. Cell 144, 719-731. PubMed PDF Supplemental Data PDF


Falk, J.E., Chan, A.C., Hoffmann, E., and Hochwagen, A. (2010). A Mec1- and PP4-dependent checkpoint couples centromere pairing to meiotic recombination. Dev Cell 19, 599-611. PubMed

Hochwagen, A., and Marais, G.A. (2010). Meiosis: a PRDM9 guide to the hotspots of recombination. Curr Biol 20, R271-274. PubMed


Hochwagen, A. (2009). Meiosis: making a synaptonemal complex just got easier. Curr Biol 19, R849-851. PubMed

Brar, G.A., Hochwagen, A., Ee, L.S., and Amon, A. (2009). The multiple roles of cohesin in meiotic chromosome morphogenesis and pairing. Mol Biol Cell 20, 1030-1047. PubMed PDF


Chen, S.Y., Tsubouchi, T., Rockmill, B., Sandler, J.S., Richards, D.R., Vader, G., Hochwagen, A., Roeder, G.S., and Fung, J.C. (2008). Global analysis of the meiotic crossover landscape. Dev Cell 15, 401-415. PubMed PDF Supplemental Data PDF

Hochwagen, A. (2008). Meiosis. Curr Biol 18, R641-R645. PubMed


Blitzblau, H.G., Bell, G.W., Rodriguez, J., Bell, S.P., and Hochwagen, A. (2007). Mapping of meiotic single-stranded DNA reveals double-stranded-break hotspots near centromeres and telomeres. Curr Biol 17, 2003-2012. PubMed


Hochwagen, A., and Amon, A. (2006). Checking your breaks: surveillance mechanisms of meiotic recombination. Curr Biol 16, R217-228. PubMed


Hochwagen, A., Tham, W.H., Brar, G.A., and Amon, A. (2005). The FK506 binding protein Fpr3 counteracts protein phosphatase 1 to maintain meiotic recombination checkpoint activity. Cell 122, 861-873. PubMed

Hochwagen, A., Wrobel, G., Cartron, M., Demougin, P., Niederhauser-Wiederkehr, C., Boselli, M.G., Primig, M., and Amon, A. (2005). Novel response to microtubule perturbation in meiosis. Mol Cell Biol 25, 4767-4781. PubMed PDF


Haering, C.H., Lowe, J., Hochwagen, A., and Nasmyth, K. (2002). Molecular architecture of SMC proteins and the yeast cohesin complex. Mol Cell 9, 773-788. PubMed


Panizza, S., Tanaka, T., Hochwagen, A., Eisenhaber, F., and Nasmyth, K. (2000). Pds5 cooperates with cohesin in maintaining sister chromatid cohesion. Curr Biol 10, 1557-1564. PubMed

Lab members


Principal investigator



Andreas Hochwagen, PhD
Principal Investigator
Assistant Professor of Biology




Viji Subramanian, PhD

Research interests: Investigating the role of the synaptonemal complex in the regulation of the double-strand break repair outcome.



Luis A. Vale Silva, PhD

Research interests: Investigating the effect of chromosome size on meiotic double-strand break formation.


Graduate Students


Tovah Markowitz
PhD student

Research interests: Understanding how condensin and cohesin control meiotic chromosome structure and recombination.



Danni Wang
PhD student

Research interests: Analyzing position effects of the repetitive rDNA array.



Andres Mansisidor
PhD student

Research interests: Investigating the structural and functional dynamics of repetitive DNA and the physiological consequences of such dynamics.



Matthew Robert Paul
PhD student

Research interests: The role of the 3D organization of chromatin on meiotic double-strand break formation.



Jonna Heldrich
PhD student

Research interests: Understanding the role of genome topology in the formation of meiotic double strand breaks.



Temistocles Molinar Jr.
PhD student

Research interests: Causes and consequences of ribosomal DNA array instability.



Neem Patel
Master's student & Technician

Research interests: Characterizing features underlying meiotic DSB end resection tracks.



Trevor Stoltzfus
Master's student

Research interests: Analysis of meiotic chromosome structure protein Hop1.



Victor A. Leon
Master's student

Research interests: Characterization of phosphorylation of the synapsis protein Zip1.



Ninkka Tamot
Master's student

Research interests: Structural and functional analysis of the high-mobility group protein Hmo1.


Undergraduate students


Vivian Utti
Undergraduate student



Michelle Shi
Undergraduate student


Former lab members


Hannah Blitzblau, PhD

Moved on to become
Senior Research Scientist at Novogy



Gerben Vader, PhD
Moved on to become
Junior Group Leader at Max Planck Institute of Molecular Physiology (Lab website)



Neil Humphryes, PhD

Moved on to become
Senior Bioinformatician at Desktop Genetics, Ltd



Marie Jezequel
Visiting Master's student



Jawaher Al-Zahrani
Research volunteer

Moved on to become
PhD student at Cornell University



Daniel Suarez
Undergraduate student

Moved on to
Postbaccalaureate program at SUNY Purchase College



Ashima Goel
Undergraduate student



Priyanka Srivastava
Undergraduate student



Anna Maria Houshangi
Master's student & Technician

Moved on to become
NYC Teaching Fellow at Department of Education



Sunny (Xiaoji) Sun
Master's student

Moved on to become
PhD Student at NYU School of Medicine



Meaghan Michelle Colletti
Master's student

Moved on to become
Student at UT Southwestern Medical Center



Daeun Hur
Undergraduate student

Moved on to become
Student at NYU College of Dentistry



Raj A. Desai
Undergraduate student

Moved on to become
Student at Columbia University College of Dental Medicine



Alisha Karim
Undergraduate student



Raghav Mishra
Undergraduate student



Namitha L Gubbi
Undergraduate student



Mihoko Tame
Lab Manager & Technician

Moved on to become
PhD student at NKI-AVL



Jill Falk
Lab Manager & Technician

Moved on to become
PhD student at MIT



Andrew Markhard
Lab Manager & Technician

Moved on to become
Technician at Mootha Lab in Broad Institute of MIT & Harvard



Genome-wide detection of meiotic DNA double-strand break hotspots using single-stranded DNA. (PDF)

BYTA Medium Recipe. (PDF)

Dissolving Benomyl in Sporulation Medium. (PDF )


Lab Photos


Click to see all photos

Contact us




Andreas Hochwagen, PhD
Principal Investigator
Assistant Professor of Biology
Lab: 1-(212)-992-6556


Lab mailing address

Hochwagen Lab
New York University
Department of Biology
1009 Silver Center
100 Washington Square East
New York, NY 10003-6688

Lab shipping address

Hochwagen Lab
New York University
Department of Biology
150 Brown
100 Washington Square East
New York, NY 10003

New York University    Department of Biology