Ph.D. in Chemistry - 2004, Yeungnam University, Korea

Spectroscopic Analysis on the porphyrin-DNA complex

 M.S. in Chemistry - 2000, Yeungnam University, Korea

Effect of cofactors and DNA on thermal denaturation 

of Rad51 and RecA protein

 Phone: (212) 998 - 8449

 Fax:      (212) 998 - 8421

 E-mail:  ya11@nyu.edu
Research: 

2005 – present: Base sequence effects on the oxidation of guanine in ODNs". Project supported by Korea Research Foundation (KRF) and New York University.

2000 - 2004: “Porphyrin-DNA Interaction”. Project supported by Korea Science and Engineering Foundation (KOSEF)

1998 - 2000: “RecA protein-DNA Interaction”. Project supported by Korea Research Foundation (KRF)

Experience in other laboratory

January 2000 – February 2000: Department of Physical Chemistry, Chalmers University of Technology, Sweden

Professor Nordén Bengt’s laboratory

Expertise:

Protein purification techniques (yeast Rad51 and E-coli RecA protein) including: Cell transformation (gene plasmid) Cell expression and growth (large scale) Electroporation Electrophoresis Column Chromatography including affinity gel, ion exchange gel FPLC, HPLC

Biophysical techniques: Absorption spectroscopy Fluorescence Spectroscopy that includes steady state/time-dependence fluorescence spectroscopy (single photon technique), anisotropy measurement and energy transfer Polarized spectroscopy (circular and linear dichroism)

DNA techniques Synthesis of fluorophore attached ss-, ds-DNA, the fluorophore being benzo[a]pyrene diol epoxide Duplex and triplex DNA hybridization Oligonucleotides synthesis and purification DNA Hot-labeling and cleavage

List of publications

  1. Cu2+-triazonan complexes conjugated with 9-aminoacridine intercalator: Their binding mode to DNA and acitvities as chemical nuclease. Youn, M.R., Lee, Y.-A., Kim, J.-M., Kim, S.K., Kim, J.H. Submitted to J. Inorg. Biochem. (Dec. 24). Revised (Feb. 24.2005)
  2. Simultaneous binding of meso-tetrakis(N-methylpyridinium-4-yl)porphyrin and 4´,6-diamidino-2 phenylindole at the minor grooves of poly(dA)·poly(dT) and poly[d(A-T)2]: Fluorescence resonance energy transfer between DNA bound drugs. Jin, B., Lee, H.M., Lee, Y.-A., Go, J.H., Kim, C., Kim, S.K. J. Am. Chem. Soc. 2005, 127, 2417-2424.
  3. Binding mode of cationic Monomer and Dimer porphyrin with Native and Synthetic Polynucleotides Studied by Polarized Light Spectroscopy Jin-Ok Kim, Young-Ae Lee, Biao Jim, Taegi Park, Rita Song, Seog K. Kim,* Biophysical Chemistry 111, 2004, 63-71.
  4. Binding of Meso-tetrakis(N-methylpyridinium-4-yl)porphyrin to AT Oligomers: Effect of Chain Length the Location of the Porphyrin Stacking Jin-Ok Kim, Young-Ae Lee, Byeong Hwa Yun, Sam Tag Kwag, Seog K. Kim Biophys. J. 2004, 86, 1012-1017.
  5. Binding of Meso-tetrakis(N-methylpyridium-4-yl)porphyrin to Triplex oligonucleotides: Evidence for the Porphyrin Stacking in the Major Groove. Young-Ae Lee, Jin-Ok Kim, Rita Song, Seog K. Kim J. Am. Chem. Soc. 2003, 125, 8106-8107.
  6. Interaction of Norfloxacin with Super-Coiled DNA. Hyŭn Jung Hwangbo, Young-Ae Lee, Jung Hag Park, Yong Rok Lee, Jong Moon Kim , Seh- Yoon Yi, Seog K. Kim Bull. Kor. Chem. Soc. 2003, 24, 579-582.
  7. Anomalous Excitonic CD of the Meso-tetrakis(3-N-methylpyridiniumyl)porphyrin Bound to Poly[d(A-T)2. Young-Ae Lee, Soomin Lee, Hyun Mee Lee, Chong-Soon Lee, Seog K. Kim J. Biochem. 2003, 133, 343-349.
  8. Binding Mode of Meso-tetrakis(N-methylpyridinium-4-yl)porphyrin to Poly[d(I-C)2]:Effect of Amino Group at the Minor Groove of Poly[d(G-C)2] on the Porphyrin-DNA Interaction. Young-Ae Lee, Soomin Lee, Tae-Sub Cho, Cheal Kim, Sung Wook Han, Seog K. Kim J. Phys. Chem. B. 2002, 106, 11351-11355.
  9. Rotation of Periphery Methylpyridine of Meso-tetrakis(n-N-methylpyridiniumyl)porphyrin (n = 2,3,4) and Its Selective Binding to Native and Synthetic DNAs. Soomin Lee, Young-Ae Lee, Hyun Me Lee, Jae Yang Lee, Seog K. Kim Biophys. J. 2002, 83, 371-381.

 

  

 

 

 

 
 
Recent Publications Projects Digital Analysis of

    Gel Images

 Instrumentation