1974 B.S., University at Albany, Biology
1977 Ph.D., University at Albany, Biology
1977-80 Amer. Cancer Soc. Postdoctoral Fellow, Rensselaer Polytechnic Institute, Chemistry

The Role of Mitochondrial Channels in Programmed Cell Death;
Molecular Studies of Two Mitochondrial Ion Channels Involved in Protein Import
This laboratory is examining the translocation of proteins across membranes. One project focuses on the fundamental mechanisms of protein import into mitochondria using electrophysiological techniques. The role of mitochondrial channels in programmed cell death is examined with a variety of additional techniques including microinjection and microsurgery of single cells, time-lapse video microscopy (fluorescence and phase), and flow cytometry. Understanding the cell death process will facilitate development of therapies that enable activation of this cascade to control the growth of malignancies and blockade of the pathway to reduce the volume of cell death after ischemic injury, e.g., stroke or heart attack.

National Institutes of Health, National Science Foundation

Priault, M., Camougrand, N., Kinnally, K.W., Vallette, F.M. and Manon, S. (2003) Yeast as a tool to study Bax/mitochondria interactions in cell death. FEMS Yeast Res. 4:15-27.

Muro C, Grigoriev SM, Pietkiewicz D, Kinnally KW, Campo ML. (2003) Comparison of the TIM and TOM Channels of the Mitochondrial Protein Import Complexes. Biophys J. 84:2981-9.

Grigoriev, S M., Jensen, R.E., Kinnally, K.W. (2003) Control of mitochondrial protein import by pH. FEBS Lett, 553:163-166.

Sabri A, Alcott SG, Elouardighi H, Pak E, Derian C, Andrade-Gordon P, Kinnally K, Steinberg SF. (2003) Neutrophil cathepsin G promotes detachment-induced cardiomyocyte apoptosis via a protease-activated receptor-independent mechanism. J Biol Chem. 278:23944-54

Khodjakov, A., Rieder, C., Mannella, C.A., Kinnally, K.W. (2004) Laser Micro-Irradiation of Mitochondria: Is there an amplified mitochondrial death signal in neural cells. Mitochondrion 3:217-227.

Guo, L. Pietkiewicz, D. Pavlov, E.V., Grigoriev, S.M., Kasianowicz, J.J., Dejean, L., Korsmeyer, S.J., Antonsson, B., Kinnally, K.W. (2004) Effects of cytochrome c on the mitochondrial apoptosis-induced channel MAC. Amer. J. Phys, Cell Phys. 286: C1109 - 1117.

Martinez-Caballero, S., Dejean, L. M., and Kinnally, K. W. (2004) Cationic amphiphillic molecules block the Mitochondrial Apoptosis-induced Channel, MAC. FEBS Lett, 568:35-38.

Grigoriev, S.M., Muro, C., Dejean, L.M., Campo, M.L., Martinez-Caballero, S., Kinnally, K.W. (2004) Electrophys. approaches to study protein translocation in mitochondria. Intn?l Rev. Cyt., Ed. K. Jeon; 238: 227-74.

E. A. Jonas, J. A. Hickman, M. Chachar, B. M. Polster, T. A. Brandt, Y. Fannjiang, I. Ivanovska, G. Basanez, K. Kinnally, J. Zimmerberg, J. M. Hardwick, and L. K. Kaczmarek (2004) Pro-apoptotic N-truncated BCL-xL protein activates VDAC mitochondrial channels in living synaptic terminals P.N.A.S. 101: 13590-13595.

Peixoto, P.M.V., Mart?nez-Caballero, S., Grigoriev, S.M., Kinnally, K.W., Campo, M. L. (2004) The ins and outs of mitochondrial channels. Recent Research Developments Biophysics 3:413-474.

Dejean LM, Martinez-Caballero S, Guo L, Hughes C, Teijido O, Ducret T, Ichas F, Korsmeyer SJ, Antonsson B, Jonas EA, Kinnally KW. (2005) Oligomeric Bax Is a Component of the Putative Cytochrome c Release Channel MAC, Mitochondrial Apoptosis-induced Channel. Mol Biol Cell 16:2424-2432.

Martinez-Caballero, S., Dejean, L.M., Jonas, E.A., and Kinnally, K.W. (2005) The role of the mitochondrial apoptosis induced channel MAC in cytochrome c release. J Bioenerg. Biomembr., 37:155-64.

Pavlov, E., Grigoriev, S.M., Dejean, L.M., Zweihorn, C.L., Mannella, C. A. and Kinnally, K. W. (2005) The mitochondrial channel VDAC has a cation-selective open state. Biochim. Biophysica Acta 1710:96-102

Frank, D.K., Szymkowiak, B., Josifovska-Chopra, O., Nakashima, T., Kinnally, K.W. (2005) Single-cell microinjection of cytochrome c resulting in gap junction?mediated apoptotic cell death of bystander cells in head and neck cancer. J. Head and Neck, 27(9):794-800.

Chinopoulos, C., Starkov, A. A., Grigoriev, S., Dejean, L. M., Kinnally, K. W., Liu, X., Ambudkar, I. S. and Fiskum, G. (2005) Diacylglycerols Activate Mitochondrial Cationic Channel(s) and Release Sequestered Ca2+. J. Bioenerg. Biomembr. 37, 229-239.

Dejean, L.M., Martinez-Caballero, S., Manon, S., and Kinnally, K.W. (2006) Regulation of the mitochondrial apoptosis induced channel MAC by Bcl-2 family proteins. Biochim. Biophysica Acta, 1762:191-201.

Kinnally, K.W., Martinez-Caballero, S., and Dejean, L.M. (2006) Detection of MAC, the mitochondrial apoptosis induced channel, and its regulation by Bcl-2 family proteins. Current Protocols in Toxicology, in press.

Dejean, L.M., Martinez-Caballero, S., and Kinnally, K.W. (2006) Is MAC the knife that cuts cytochrome c from mitochondria during apoptosis? Cell Death and Differentiation, in press.