1965 B.S., Brooklyn College
1970 M.S., Brandeis University
1970 Ph.D., Brandeis University
1969-71 Postdoc Max Planck Institute for Biophysical Chemistry, Gottingen
1971-73 Postdoc University of California, Berkeley

• Effects of nutrients on spontaneous and chemically induced mutagenesis in lacI transgenic mice. Funded by NIH.
• Mutagenesis by smokeless tobacco, tobacco components, and tobacco carcinogens in lacZ transgenic mice. Funded by smokeless Tobacco Research Council
• Detection of chemically modified DNA in human oral tissue.
• Metabolism of tobacco carcinogens by human oral tissue.
• Development of sensitive fluorescent assays to detect DNA damage.

Chemical Carcinogenesis and Mutagenesis
Research in my laboratory focuses on measuring mutations in tissues of lacZ transgenic mice, chemoprevention of mutagenesis, development of high sensitivity fluorescence assays for detection of modified nucleotides from DNA, the metabolism of carcinogens, interactions of carcinogens with DNA, detection of carcinogen-DNA adducts, and determination of mutational spectra of carcinogens. Laboratory methodologies include high performance liquid chromatography, mutagenesis, colony hybridization probing, enzyme assays, DNA sequencing, and 32P-post-labelling.

NIH (2 projects), Smokeless Tobacco Research Council

Guttenplan, J.B. Different mutagenic metabolites from N-nitrosomethylurea and N-nitrosodimethylamine. Carcinogenesis, 14:1013-1019, 1993.

Guttenplan, J.B., Kokkinakis, D. High mutagenic activity of N-nitroso-bis- (2-oxopropyl) amine and N-nitroso-bis-2-(hydroxypropylamine) in the hamster liver host-mediated assay. Carcinogenesis, 14 (1993): 1621-1625.

Chung, F.L., Chen, H. J.C., Guttenplan, J.B., Nishikawa. 2, 3-epoxy-4-hydroxynonanal as a potential initiating agent of lipid peroxidation. Carcinogenesis, 14 (1993): 2073-2077.

Jiao, J., Guttenplan, J.B., Glickman, B.W., Anderson, M.W., Xin, L-Y, Zielenska, M. Mutational specificities of environmental carcinogens in the lacI gene of E. coli VII: The host-mediated assay and its comparison with in vitro mutagenesis induced by 4- (methylnitrosamino) -1- (3-pyridyl)-1-butanone. Mol. Carcinogenesis, 8 (1993): 127-131.

Tinwell, H., Paton, D., Guttenplan, J.B., Ashby, J. The unexpected genetic toxicity to rodents of the N', N'- dimethyl analogues of MU and ENU. Environ. Mol. Mutagen., 27 (1996): 202-211.

Nath, R., Ocando, J.E., Guttenplan, J.B., and Chung, F.-L. Propanodeoxyguanosine adducts: Potential new biomarkers of smoking induced DNA damage in human oral tissue. Cancer Res., 58 (1998): 581-584.

Bhaumik, M., Harris, T., Johnson, L., Guttenplan, J.B., Rogler, C., and Stanley, P. Progression of chemically induced hepatoma is severely retarded in mice lacking GlcNAc on N-glycans. Cancer Res., 58 (1998): 2881-2887.

Kosinska, W., von Pressentin, M.d.M. and Guttenplan, J.B. Comparative mutagenicity of benzo(a)pyrene in breast and oral tissues of lacZ mice. Carcinogenesis, 20 (1999): 2167-2170.

Kosinska, W., von Pressentin, M.d.M., El Bayoumy, K. and Guttenplan, J.B., Mutagenesis by 4- Nitroquinoline-N-oxide in Oral Cavity and Esophagus in lacZ Mice and Effects of Organic Selenium Mutation Research, Mutation Res. 466 (2000): 71-78.

Kosinska, W., von Pressentin, M.d.M. and Guttenplan, J.B. Mutagenesis by Tobacco-Specific Nitrosamines in lacZ Mice Upper Aerodigestive Tissue and Inhibition by Green Tea, Carcinogenesis, 21, 203-206 (2000).

Guttenplan, J.B., Chen, M., Kosinska, W., Zhau,Z and Cohen, L. Effects of Dietary Lycopene on Spontaneous and Benzo(a)pyrene-Induced Mutagenesis in the lacZ Mouse. Cancer Letters, 164, 1-6 (2001).

Guttenplan, J.B., Kosinska, W., von Pressentin, M.d.M., Rosa, J, El-Bayoumy, K., Effects of 1,4-Phenylenebis(methylene)selenocyanate (p-XSC) and Vitamin E on 4 nitroquinoline-N-oxide (4-NQO)-Induced Mutagenesis in lacZ Mouse Upper Aerodigestive Tissue. Mutation Res., in press.

Kosinska, W., Pelle, E, von Pressentin, M.d.M., Chen, M., Joseph B. Guttenplan, J.B. Comparative Mutagenicities of Bleomycin and Ferric-Nitrilotriacetate in lacZ Mice. Cancer Letters, in press.